pF25A ICE T7 Flexi(R) Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pF25A ICE T7 Flexi(R) Vector sequence reference points: Base pairs 3947 T7 RNA polymerase promoter 21-39 5' polyhedrin UTR 40-85 Sgf I region 86-93 Barnase coding region 117-452 Pme I region 454-461 3' polyhedrin UTR 610-983 Synthetic poly(A) signal 988-1017 T7 terminator 1018-1065 Beta-lactamase (Ampr) coding region 1446-2306 ColE1-derived plasmid origin of replication 2461-2497 cer region (for E. coli XerCD recombinase) 3168-3453 .. 1 GGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGA GTATTTTTAT 51 TCTTTCGTAA AAAAATTAGA AAAATAAAAT ATAAAGCGAT CGCCATGGAA 101 TAAGTAAGGA ATCCACATGG CACAGGTTAT CAACACGTTT GACGGGGTTG 151 CGGATTATCT TCAGACATAT CATAAGCTAC CTGATAATTA CATTACAAAA 201 TCAGAAGCAC AAGCCCTCGG CTGGGTGGCA TCAAAAGGGA ACCTTGCAGA 251 CGTCGCTCCG GGGAAAAGCA TCGGCGGAGA CATCTTCTCA AACAGGGAAG 301 GCAAACTCCC GGGCAAAAGC GGACGAACAT GGCGTGAAGC GGATATTAAC 351 TATACATCAG GCTTCAGAAA TTCAGACCGG ATTCTTTACT CAAGCGACTG 401 GCTGATTTAC AAAACAACGG ACCATTATCA GACCTTTACA AAAATCAGAT 451 AATGTTTAAA CGAATTCGGG CTCGGTACCC GGGGATCCTC TAGAGTCGAC 501 CTGCAGGCAT GCAAGCTGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT 551 GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CTAGAGTCGG 601 GCGGCTGTAA AACACGATAC ATTGTTATTA GTACATTTAT TAAGCGCTAG 651 ATTCTGTGCG TTGTTGATTT ACAGACAATT GTTGTACGTA TTTTAATAAT 701 TCATTAAATT TATAATCTTT AGGGTGGTAT GTTAGAGCGA AAATCAAATG 751 ATTTTCAGCG TCTTTATATC TGAATTTAAA TATTAAATCC TCAATAGATT 801 TGTAAAATAG GTTTCGATTA GTTTCAAACA AGGGTTGTTT TTCCGAACCG 851 ATGGCTGGAC TATCTAATGG ATTTTCGCTC AACGCCACAA AACTTGCCAA 901 ATCTTGTAGC AGCAATCTAG CTTTGTCGAT ATTCGTTTGT GTTTTGTTTT 951 GTAATAAAGG TTCGACGTCG TTCAAAATAT TATCCGCAAA AAAAAAAAAA 1001 AAAAAAAAAA AAAAAAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC 1051 TTGAGGGGTT TTTTGCTCGC GAGCGGCCGC AGGCCTCTAA ACGGGTCTTG 1101 AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGTTCGC TTGCTGTCCA 1151 TAAAACCGCC CAGTCTAGCT ATCGCCATGT AAGCCCACTG CAAGCTACCT 1201 GCTTTCTCTT TGCGCTTGCG TTTTCCCTTG TCCAGATAGC CCAGTAGCTG 1251 ACATTCATCC GGGGTCAGCA CCGTTTCTGC GGACTGGCTT TCTACGTAAT 1301 GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC 1351 CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA 1401 CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG 1451 TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC 1501 TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA 1551 GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG 1601 TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA 1651 CTTTCAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG 1701 CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA 1751 GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG 1801 AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA 1851 CTTCTGACAA CTATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA 1901 CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG 1951 AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA 2001 ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG 2051 GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC 2101 TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC 2151 GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA 2201 GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG 2251 ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT 2301 TGGTAATTCG AAATGACCGA CCAAGCGACG CCCAACCGGT ATCAGCTCAC 2351 TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 2401 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG 2451 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA 2501 AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 2551 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC 2601 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG 2651 CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 2701 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG 2751 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA 2801 TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 2851 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA 2901 GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA 2951 AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 3001 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATTTC 3051 AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA 3101 AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 3151 CTAGATCCTT TTATAGTCCG GAAATACAGG AACGCACGCT GGATGGCCCT 3201 TCGCTGGGAT GGTGAAACCA TGAAAAATGG CAGCTTCAGT GGATTAAGTG 3251 GGGGTAATGT GGCCTGTACC CTCTGGTTGC ATAGGTATTC ATACGGTTAA 3301 AATTTATCAG GCGCGATTGC GGCAGTTTTT CGGGTGGTTT GTTGCCATTT 3351 TTACCTGTCT GCTGCCGTGA TCGCGCTGAA CGCGTTTTAG CGGTGCGTAC 3401 AATTAAGGGA TTATGGTAAA TCCACTTACT GTCTGCCCTC GTAGCCATCG 3451 AGATAAACCG CAGTACTCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG 3501 ATCTTTTCAG CCTGATACAG ATTAAATCAG AACGCAGAAG CGGTCTGATA 3551 AAACAGAATT TGCCTGGCGG CAGTAGCGCG GTGGTCCCAC CTGACCCCAT 3601 GCCGAACTCA GAAGTGAAAC GCCGTAGCGC CGATGGTAGT GTGGGGTCTC 3651 CCCATGCGAG AGTAGGGAAC TGCCAGGCAT CAAATAAAAC GAAAGGCTCA 3701 GTCGAAAGAC TGGGCCTTTC GTTTTATCTG TTGTTTGTCG GTGAACGCTC 3751 TCCTGAGTAG GACAAATCCG CCGGGAGCGG ATTTGAACGT TGCGAAGCAA 3801 CGGCCCGGAG GGTGGCGGGC AGGACGCCCG CCATAAACTG CCAGGCATCA 3851 AATTAAGCAG AAGGCCATCC TGACGGATGG CCTTTTTGCG TTTCTACAAA 3901 CTCTTTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCAT Sequence file prepared 11-Aug-08.