This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pFC32A Nluc CMV-Hygro Flexi(R) Vector sequence reference points: Base pairs 6299 CMV immediate early enhancer/promoter 1–742 Chimeric intron 857–989 T7 RNA polymerase promoter (–17 to +3) 1033–1052 SgfI 1056–1063 Barnase coding region 1087–1422 EcoICRI 1442–1447 Linker 1447–1461 NanoLuc® protein coding region 1462–1971 SV40 late poly(A) signal 2108–2329 SV40 early enhancer/promoter 2428–2840 Synthetic hygromycin (Hygr) coding region 2871–3908 Synthetic poly(A) signal 3932–3980 β-lactamase (Ampr) coding region 4241–5101 ColE1-derived plasmid origin of replication 5256–5292 1 TCAATATTGG CCATTAGCCA TATTATTCAT TGGTTATATA GCATAAATCA 51 ATATTGGCTA TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC 101 ATTTATATTG GCTCATGTCC AATATGACCG CCATGTTGGC ATTGATTATT 151 GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT 201 ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA 251 CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT 301 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC 351 GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG 401 CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA 451 GTACATGACC TTACGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG 501 TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC CAATGGGCGT 551 GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT 601 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC 651 GTAATAACCC CGCCCCGTTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG 701 GAGGTCTATA TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCACTAGAAG 751 CTTTATTGCG GTAGTTTATC ACAGTTAAAT TGCTAACGCA GTCAGTGCTT 801 CTGACACAAC AGTCTCGAAC TTAAGCTGCA GAAGTTGGTC GTGAGGCACT 851 GGGCAGGTAA GTATCAAGGT TACAAGACAG GTTTAAGGAG ACCAATAGAA 901 ACTGGGCTTG TCGAGACAGA GAAGACTCTT GCGTTTCTGA TAGGCACCTA 951 TTGGTCTTAC TGACATCCAC TTTGCCTTTC TCTCCACAGG TGTCCACTCC 1001 CAGTTCAATT ACAGCTCTTA AGGCTAGAGT ATTAATACGA CTCACTATAG 1051 GGCTAGCGAT CGCCATGGAA TAAGTAAGGA ATCCACATGG CACAGGTTAT 1101 CAACACGTTT GACGGGGTTG CGGATTATCT TCAGACATAT CATAAGCTAC 1151 CTGATAATTA CATTACAAAA TCAGAAGCAC AAGCCCTCGG CTGGGTGGCA 1201 TCAAAAGGGA ACCTTGCAGA CGTCGCTCCG GGGAAAAGCA TCGGCGGAGA 1251 CATCTTCTCA AACAGGGAAG GCAAACTCCC GGGCAAAAGC GGACGAACAT 1301 GGCGTGAAGC GGATATTAAC TATACATCAG GCTTCAGAAA TTCAGACCGG 1351 ATTCTTTACT CAAGCGACTG GCTGATTTAC AAAACAACGG ACCATTATCA 1401 GACCTTTACA AAAATCAGAT AATGTTTAAT GACCCCGTGT CGAGCTCTCG 1451 GCTCGAGCGG CGTCTTCACA CTCGAAGATT TCGTTGGGGA CTGGCGACAG 1501 ACAGCCGGCT ACAACCTGGA CCAAGTCCTT GAACAGGGAG GTGTGTCCAG 1551 TTTGTTTCAG AATCTCGGGG TGTCCGTAAC TCCGATCCAA AGGATTGTCC 1601 TGAGCGGTGA AAATGGGCTG AAGATCGACA TCCATGTCAT CATCCCGTAT 1651 GAAGGTCTGA GCGGCGACCA AATGGGCCAG ATCGAAAAAA TTTTTAAGGT 1701 GGTGTACCCT GTGGATGATC ATCACTTTAA GGTGATCCTG CACTATGGCA 1751 CACTGGTAAT CGACGGGGTT ACGCCGAACA TGATCGACTA TTTCGGACGG 1801 CCGTATGAAG GCATCGCCGT GTTCGACGGC AAAAAGATCA CTGTAACAGG 1851 GACCCTGTGG AACGGCAACA AAATTATCGA CGAGCGCCTG ATCAACCCCG 1901 ACGGCTCCCT GCTGTTCCGA GTAACCATCA ACGGAGTGAC CGGCTGGCGG 1951 CTGTGCGAAC GCATTCTGGC GTAAGGCCGC GACTCTAGAG TCGACCTGCA 2001 GGCATGCAAG CTGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT 2051 GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCGGCCG 2101 CTTCGAGCAG ACATGATAAG ATACATTGAT GAGTTTGGAC AAACCACAAC 2151 TAGAATGCAG TGAAAAAAAT GCTTTATTTG TGAAATTTGT GATGCTATTG 2201 CTTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAA CAACAACAAT 2251 TGCATTCATT TTATGTTTCA GGTTCAGGGG GAGATGTGGG AGGTTTTTTT 2301 AAGCAAGTAA AACCTCTACA AATGTGGTAA AATCGAATTT TAACAAAATA 2351 TTAACGCTTA CAATTTCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC 2401 GGTATTTCAC ACCGCATACG CGGATCTGCG CAGCACCATG GCCTGAAATA 2451 ACCTCTGAAA GAGGAACTTG GTTAGGTACC TTCTGAGGCG GAAAGAACCA 2501 GCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG 2551 CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT 2601 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT 2651 CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC 2701 TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT 2751 TTTATTTATG CAGAGGCCGA GGCCGCCTCG GCCTCTGAGC TATTCCAGAA 2801 GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTCGATT 2851 CTTCTGACAC TAGCGCCACC ATGAAGAAGC CCGAACTCAC CGCTACCAGC 2901 GTTGAAAAAT TTCTCATCGA GAAGTTCGAC AGTGTGAGCG ACCTGATGCA 2951 GTTGTCGGAG GGCGAAGAGA GCCGAGCCTT CAGCTTCGAT GTCGGCGGAC 3001 GCGGCTATGT ACTGCGGGTG AATAGCTGCG CTGATGGCTT CTACAAAGAC 3051 CGCTACGTGT ACCGCCACTT CGCCAGCGCT GCACTACCCA TCCCCGAAGT 3101 GTTGGACATC GGCGAGTTCA GCGAGAGCCT GACATACTGC ATCAGTAGAC 3151 GCGCCCAAGG CGTTACTCTC CAAGACCTCC CCGAAACAGA GCTGCCTGCT 3201 GTGTTACAGC CTGTCGCCGA AGCTATGGAT GCTATTGCCG CCGCCGACCT 3251 CAGTCAAACC AGCGGCTTCG GCCCATTCGG GCCCCAAGGC ATCGGCCAGT 3301 ACACAACCTG GCGGGATTTC ATTTGCGCCA TTGCTGATCC CCATGTCTAC 3351 CACTGGCAGA CCGTGATGGA CGACACCGTG TCCGCCAGCG TAGCTCAAGC 3401 CCTGGACGAA CTGATGCTGT GGGCCGAAGA CTGTCCCGAG GTGCGCCACC 3451 TCGTCCATGC CGACTTCGGC AGCAACAACG TCCTGACCGA CAACGGCCGC 3501 ATCACCGCCG TAATCGACTG GTCCGAAGCT ATGTTCGGGG ACAGTCAGTA 3551 CGAGGTGGCC AACATCTTCT TCTGGCGGCC CTGGCTGGCT TGCATGGAGC 3601 AGCAGACTCG CTACTTCGAG CGCCGGCATC CCGAGCTGGC CGGCAGCCCT 3651 CGTCTGCGAG CCTACATGCT GCGCATCGGC CTGGATCAGC TCTACCAGAG 3701 CCTCGTGGAC GGCAACTTCG ACGATGCTGC CTGGGCTCAA GGCCGCTGCG 3751 ATGCCATCGT CCGCAGCGGG GCCGGCACCG TCGGTCGCAC ACAAATCGCT 3801 CGCCGGAGCG CAGCCGTATG GACCGACGGC TGCGTCGAGG TGCTGGCCGA 3851 CAGCGGCAAC CGCCGGCCCA GTACACGACC GCGCGCTAAG GAGGTAGGTC 3901 GAGTTTAGAC TCTAGCCATC ACGATGGCCG CAATAAAATA TCTTTATTTT 3951 CATTACATCT GTGTGTTGGT TTTTTGTGTG AATCGATAGC GATAAGGATC 4001 CTCTTTGCGC TTGCGTTTTC CCTTGTCCAG ATAGCCCAGT AGCTGACATT 4051 CATCCGGGGT CAGCACCGTT TCTGCGGACT GGCTTTCTAC GTAATGGTTT 4101 CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT 4151 TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA 4201 ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC 4251 AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT 4301 GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA 4351 GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA 4401 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTC 4451 AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA 4501 GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT 4551 CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA 4601 TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT 4651 GACAACTATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG 4701 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC 4751 ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC 4801 GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC 4851 AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC 4901 TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA 4951 GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT 5001 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA 5051 CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA 5101 ATTCGAAATG ACCGACCAAG CGACGCCCAA CCGGTATCAG CTCACTCAAA 5151 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA 5201 TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG 5251 CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 5301 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG 5351 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG 5401 CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 5451 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA 5501 AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA 5551 TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 5601 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG 5651 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG 5701 ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 5751 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT 5801 TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATTTCAAGAA 5851 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 5901 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA 5951 TCCTTTTATA GTCCGGAAAT ACAGGAACGC ACGCTGGATG GCCCTTCGCT 6001 GGGATGGTGA AACCATGAAA AATGGCAGCT TCAGTGGATT AAGTGGGGGT 6051 AATGTGGCCT GTACCCTCTG GTTGCATAGG TATTCATACG GTTAAAATTT 6101 ATCAGGCGCG ATTGCGGCAG TTTTTCGGGT GGTTTGTTGC CATTTTTACC 6151 TGTCTGCTGC CGTGATCGCG CTGAACGCGT TTTAGCGGTG CGTACAATTA 6201 AGGGATTATG GTAAATCCAC TTACTGTCTG CCCTCGTAGC CATCGAGATA 6251 AACCGCAGTA CTCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCT Sequence file created 5-December-2013.