BTK-NanoLucŪ Fusion Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 BTK sequence reference points: Base pairs 6720 CMV immediate-early enhancer/promoter 1-742 chimeric intron 857-989 T7 RNA Polymerase promoter (-17 to +3) 1033-1052 SgfI Site 1056-1063 BTK-NanoLucŪ Fusion Coding Region 1065-3575 Pme I EcoICRI Chimeric Site 1151-1157 EcoICR I 2133-2138 Pme I EcoICRI Chimeric Site 3042-3048 C-terminal NanoLuc 3063-3572 Sv40 late polyadenylation signal 3709-3930 SV40 enhancer and early promoter 4029-4447 SV40 Enhancer 4102-4338 (Reverse) Sv40 Min Ori 4345-4410 EM7 bacterial promoter 4455-4521 Neo-Kan Resistance 4535-5329 Synthetic PolyAdenylation Signal Sequence 5393-5441 ColE1-derived plasmid replication origin 5677-5713 ---- 1 TCAATATTGG CCATTAGCCA TATTATTCAT TGGTTATATA GCATAAATCA 51 ATATTGGCTA TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC 101 ATTTATATTG GCTCATGTCC AATATGACCG CCATGTTGGC ATTGATTATT 151 GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT 201 ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA 251 CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT 301 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC 351 GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG 401 CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA 451 GTACATGACC TTACGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG 501 TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC CAATGGGCGT 551 GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT 601 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC 651 GTAATAACCC CGCCCCGTTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG 701 GAGGTCTATA TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCACTAGAAG 751 CTTTATTGCG GTAGTTTATC ACAGTTAAAT TGCTAACGCA GTCAGTGCTT 801 CTGACACAAC AGTCTCGAAC TTAAGCTGCA GAAGTTGGTC GTGAGGCACT 851 GGGCAGGTAA GTATCAAGGT TACAAGACAG GTTTAAGGAG ACCAATAGAA 901 ACTGGGCTTG TCGAGACAGA GAAGACTCTT GCGTTTCTGA TAGGCACCTA 951 TTGGTCTTAC TGACATCCAC TTTGCCTTTC TCTCCACAGG TGTCCACTCC 1001 CAGTTCAATT ACAGCTCTTA AGGCTAGAGT ATTAATACGA CTCACTATAG 1051 GGCTAGCGAT CGCCATGGCC GCAGTGATTC TGGAGAGCAT CTTTCTGAAG 1101 CGATCCCAAC AGAAAAAGAA AACATCACCT CTAAACTTCA AGAAGCGCCT 1151 GTTTCTCTTG ACCGTGCACA AACTCTCCTA CTATGAGTAT GACTTTGAAC 1201 GTGGGAGAAG AGGCAGTAAG AAGGGTTCAA TAGATGTTGA GAAGATCACT 1251 TGTGTTGAAA CAGTGGTTCC TGAAAAAAAT CCTCCTCCAG AAAGACAGAT 1301 TCCGAGAAGA GGTGAAGAGT CCAGTGAAAT GGAGCAAATT TCAATCATTG 1351 AAAGGTTCCC TTATCCCTTC CAGGTTGTAT ATGATGAAGG GCCTCTCTAC 1401 GTCTTCTCCC CAACTGAAGA ACTAAGGAAG CGGTGGATTC ACCAGCTCAA 1451 AAACGTAATC CGGTACAACA GTGATCTGGT TCAGAAATAT CACCCTTGCT 1501 TCTGGATCGA TGGGCAGTAT CTCTGCTGCT CTCAGACAGC CAAAAATGCT 1551 ATGGGCTGCC AAATTTTGGA GAACAGGAAT GGAAGCTTAA AACCTGGGAG 1601 TTCTCACCGG AAGACAAAAA AGCCTCTTCC CCCAACGCCT GAGGAGGACC 1651 AGATCTTGAA AAAGCCACTA CCGCCTGAGC CAGCAGCAGC ACCAGTCTCC 1701 ACAAGTGAGC TGAAAAAGGT TGTGGCCCTT TATGATTACA TGCCAATGAA 1751 TGCAAATGAT CTACAGCTGC GGAAGGGTGA TGAATATTTT ATCTTGGAGG 1801 AAAGCAACTT ACCATGGTGG AGAGCACGAG ATAAAAATGG GCAGGAAGGC 1851 TACATTCCTA GTAACTATGT CACTGAAGCA GAAGACTCCA TAGAAATGTA 1901 TGAGTGGTAT TCCAAACACA TGACTCGGAG TCAGGCTGAG CAACTGCTAA 1951 AGCAAGAGGG GAAAGAAGGA GGTTTCATTG TCAGAGACTC CAGCAAAGCT 2001 GGCAAATATA CAGTGTCTGT GTTTGCTAAA TCCACAGGGG ACCCTCAAGG 2051 GGTGATACGT CATTATGTTG TGTGTTCCAC ACCTCAGAGC CAGTATTACC 2101 TGGCTGAGAA GCACCTTTTC AGCACCATCC CTGAGCTCAT TAACTACCAT 2151 CAGCACAACT CTGCAGGACT CATATCCAGG CTCAAATATC CAGTGTCTCA 2201 ACAAAACAAG AATGCACCTT CCACTGCAGG CCTGGGATAC GGATCATGGG 2251 AAATTGATCC AAAGGACCTG ACCTTCTTGA AGGAGCTGGG GACTGGACAA 2301 TTTGGGGTAG TGAAGTATGG GAAATGGAGA GGCCAGTACG ACGTGGCCAT 2351 CAAGATGATC AAAGAAGGCT CCATGTCTGA AGATGAATTC ATTGAAGAAG 2401 CCAAAGTCAT GATGAATCTT TCCCATGAGA AGCTGGTGCA GTTGTATGGC 2451 GTCTGCACCA AGCAGCGCCC CATCTTCATC ATCACTGAGT ACATGGCCAA 2501 TGGCTGCCTC CTGAACTACC TGAGGGAGAT GCGCCACCGC TTCCAGACTC 2551 AGCAGCTGCT AGAGATGTGC AAGGATGTCT GTGAAGCCAT GGAATACCTG 2601 GAGTCAAAGC AGTTCCTTCA CCGAGACCTG GCAGCTCGAA ACTGTTTGGT 2651 AAACGATCAA GGAGTTGTTA AAGTATCTGA TTTCGGCCTG TCCAGGTATG 2701 TCCTGGATGA TGAATACACA AGCTCAGTAG GCTCCAAATT TCCAGTCCGG 2751 TGGTCCCCAC CGGAAGTCCT GATGTATAGC AAGTTCAGCA GCAAATCTGA 2801 CATTTGGGCT TTTGGGGTTT TGATGTGGGA AATTTACTCC CTGGGGAAGA 2851 TGCCATATGA GAGATTTACT AACAGTGAGA CTGCTGAACA CATTGCCCAA 2901 GGCCTACGTC TCTACAGGCC TCATCTGGCT TCAGAGAAGG TATATACCAT 2951 CATGTACAGT TGTTGGCATG AGAAAGCAGA TGAGCGTCCC ACTTTCAAAA 3001 TTCTTCTGAG CAATATTCTA GATGTCATGG ATGAAGAAAG CGTTTCTCTC 3051 GGCTCGAGCG GCGTCTTCAC ACTCGAAGAT TTCGTTGGGG ACTGGCGACA 3101 GACAGCCGGC TACAACCTGG ACCAAGTCCT TGAACAGGGA GGTGTGTCCA 3151 GTTTGTTTCA GAATCTCGGG GTGTCCGTAA CTCCGATCCA AAGGATTGTC 3201 CTGAGCGGTG AAAATGGGCT GAAGATCGAC ATCCATGTCA TCATCCCGTA 3251 TGAAGGTCTG AGCGGCGACC AAATGGGCCA GATCGAAAAA ATTTTTAAGG 3301 TGGTGTACCC TGTGGATGAT CATCACTTTA AGGTGATCCT GCACTATGGC 3351 ACACTGGTAA TCGACGGGGT TACGCCGAAC ATGATCGACT ATTTCGGACG 3401 GCCGTATGAA GGCATCGCCG TGTTCGACGG CAAAAAGATC ACTGTAACAG 3451 GGACCCTGTG GAACGGCAAC AAAATTATCG ACGAGCGCCT GATCAACCCC 3501 GACGGCTCCC TGCTGTTCCG AGTAACCATC AACGGAGTGA CCGGCTGGCG 3551 GCTGTGCGAA CGCATTCTGG CGTAAGGCCG CGACTCTAGA GTCGACCTGC 3601 AGGCATGCAA GCTGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT 3651 TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCGGCC 3701 GCTTCGAGCA GACATGATAA GATACATTGA TGAGTTTGGA CAAACCACAA 3751 CTAGAATGCA GTGAAAAAAA TGCTTTATTT GTGAAATTTG TGATGCTATT 3801 GCTTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA ACAACAACAA 3851 TTGCATTCAT TTTATGTTTC AGGTTCAGGG GGAGATGTGG GAGGTTTTTT 3901 TAAGCAAGTA AAACCTCTAC AAATGTGGTA AAATCGAATT TTAACAAAAT 3951 ATTAACGCTT ACAATTTCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG 4001 CGGTATTTCA CACCGCATAC GCGGATCTGC GCAGCACCAT GGCCTGAAAT 4051 AACCTCTGAA AGAGGAACTT GGTTAGGTAC CTTCTGAGGC GGAAAGAACC 4101 AGCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA 4151 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG 4201 TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC 4251 TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC 4301 CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT 4351 TTTTATTTAT GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG CTATTCCAGA 4401 AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTTAAT 4451 TAACTGTTGA CAATTAATCA TCGGCATAGT ATATCGGCAT AGTATAATAC 4501 GACAAGGTGA GGAACTAAAC CCAGGAGGCA GATCATGATT GAACAAGATG 4551 GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT 4601 GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT 4651 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG 4701 CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG 4751 ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG 4801 GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC 4851 ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG 4901 CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA 4951 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG 5001 ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC 5051 AGGCTCAAGG CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG 5101 CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT 5151 TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA GGACATAGCG 5201 TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG 5251 CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT 5301 TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA 5351 TGACCGACCA AGCGACGCCC AACCTGCCAT CACGATGGCC GCAATAAAAT 5401 ATCTTTATTT TCATTACATC TGTGTGTTGG TTTTTTGTGT GAATCGATAG 5451 CGATAAGGAT CCTCTTTGCG CTTGCGTTTT CCCTTGTCCA GATAGCCCAG 5501 TAGCTGACAT TCATCCGGGG TCAGCACCGT TTCTGCGGAC TGGCTTTCTA 5551 CCCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG 5601 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG 5651 AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 5701 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA 5751 CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC 5801 TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 5851 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT 5901 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT 5951 CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 6001 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA 6051 GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT 6101 AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA 6151 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA 6201 CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC 6251 AGAAAAAAAG GATTTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 6301 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT 6351 CAAAAAGGAT CTTCACCTAG ATCCTTTTAT AGTCCGGAAA TACAGGAACG 6401 CACGCTGGAT GGCCCTTCGC TGGGATGGTG AAACCATGAA AAATGGCAGC 6451 TTCAGTGGAT TAAGTGGGGG TAATGTGGCC TGTACCCTCT GGTTGCATAG 6501 GTATTCATAC GGTTAAAATT TATCAGGCGC GATTGCGGCA GTTTTTCGGG 6551 TGGTTTGTTG CCATTTTTAC CTGTCTGCTG CCGTGATCGC GCTGAACGCG 6601 TTTTAGCGGT GCGTACAATT AAGGGATTAT GGTAAATCCA CTTACTGTCT 6651 GCCCTCGTAG CCATCGAGAT AAACCGCAGT ACTCCGGCCA CGATGCGTCC 6701 GGCGTAGAGG ATCGAGATCT Sequence file prepared 3/7/2018 by Feature database and feature finder version 1.0.5.0.